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Proceedings Paper

CdTe/CdS-MPA quantum dots as fluorescent probes to label yeast cells: synthesis, characterization and conjugation with Concanavalin A
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Paper Abstract

Candida albicans is the most frequent human opportunistic pathogenic fungus and one of the most important causes of nosocomial infections. In fact, diagnosis of invasive candidiasis presents unique problems. The aim of this work was to evaluate, by fluorescence image analysis, cellular labeling of C. albicans with CdTe/CdS quantum dots conjugated or not to concanavalin A (ConA). Yeast cells were incubated with CdTe/CdS quantum dots (QD) stabilized with mercaptopropionic acid (MPA) (emission peak at 530 nm) for 1 hour. In the overall study we observed no morphological alterations. The fluorescence microscopic analysis of the yeast cells showed that the non-functionalized QDs do not label C. albicans cells, while for the QD conjugated to ConA the cells showed a fluorescence profile indicating that the membrane was preferentially marked. This profile was expected since Concanavalin A is a protein that binds specifically to terminal carbohydrate residues at the membrane cell surface. The results suggest that the QD-labeled Candida cells represent a promising tool to open new possibilities for a precise evaluation of fungal infections in pathological conditions.

Paper Details

Date Published: 2 February 2012
PDF: 6 pages
Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320D (2 February 2012); doi: 10.1117/12.909060
Show Author Affiliations
Ilka T. Kato, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Camila C. Santos, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Endi Benetti, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Denise P. L. A. Tenório, Univ. Federal de Pernambuco (Brazil)
Paulo E. Cabral Filho, Univ. Federal de Pernambuco (Brazil)
Caetano P. Sabino, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Adriana Fontes, Univ. Federal de Pernambuco (Brazil)
Beate S. Santos, Univ. Federal de Pernambuco (Brazil)
Renato A. Prates, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Martha S. Ribeiro, Instituto de Pesquisas Energéticas e Nucleares (Brazil)


Published in SPIE Proceedings Vol. 8232:
Colloidal Nanocrystals for Biomedical Applications VII
Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski, Editor(s)

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