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Proceedings Paper

Probing the tumor microenvironment: collection and induction
Author(s): James K. Williams; Michael R. Padgen; Yarong Wang; David Entenberg; Frank Gertler; John S. Condeelis; James Castracane
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Paper Abstract

The Nano Intravital Device, or NANIVID, is under development as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a hydrogel blend that contains growth factors or other chemicals to be delivered to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel will swell and release these entrapped molecules, forming a gradient. Validation of the device has been performed in vitro using epidermal growth factor (EGF) and MenaINV, a highly invasive, rat mammary adenocarcinoma cell line. In both 2-D and 3-D environments, cells migrated toward the gradient of EGF released from the device. The chorioallantoic membrane (CAM) of White Leghorn chicken eggs is being utilized to grow xenograft tumors that will be used for ex vivo cell collection. Device optimization is being performed for in vivo use as a tool to collect the invasive cell population. Preliminary cell collection experiments in vivo were performed using a mouse model of breast cancer. As a second application, the device is being explored as a delivery vehicle for chemicals that induce controlled changes in the tumor microenvironment. H2O2 was loaded in the device and generated intracellular reactive oxygen species (ROS) in cells near the device outlet. In the future, other induction targets will be explored, including hypoglycemia and the manipulation of extracellular matrix stiffness.

Paper Details

Date Published: 14 February 2012
PDF: 7 pages
Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 825105 (14 February 2012); doi: 10.1117/12.909045
Show Author Affiliations
James K. Williams, Univ. at Albany (United States)
Michael R. Padgen, Univ. at Albany (United States)
Yarong Wang, Yeshiva Univ. (United States)
David Entenberg, Yeshiva Univ. (United States)
Frank Gertler, Massachusetts Institute of Technology (United States)
John S. Condeelis, Yeshiva Univ. (United States)
James Castracane, Univ. at Albany (United States)


Published in SPIE Proceedings Vol. 8251:
Microfluidics, BioMEMS, and Medical Microsystems X
Holger Becker; Bonnie L. Gray, Editor(s)

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