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Proceedings Paper

Photoacoustic imaging of chemotherapy-induced apoptosis in squamous cell carcinoma
Author(s): Quihong Yang; Huizhong Cui; Shuang Cai; Xinmai Yang; M. Laird Forrest
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Paper Abstract

Anti-cancer drugs typically exert their pharmacological effect on tumors by inducing apoptosis, or programmed cell death, within the cancer cells, with PCD occurring as soon as 4 hours after treatment. Detection of apoptosis in patients could decisively report a response to treatment days or even weeks before MRI, CAT, and ultrasound indicate morphological changes in the tumor. Here we developed a novel near-infrared dye based imaging probe to directly detect apoptosis with high specificity in cancer cells by utilizing a non-invasive photoacoustic imaging technique. Nude mice bearing head and neck tumors received cisplatin chemotherapy were imaged by PAI after tail vein injection of the contrast agent. In vivo PAI indicated a strong apoptotic response to chemotherapy on the peripheral margins of tumors, whereas untreated controls showed no contrast enhancement by PAI. The apoptotic status of the mouse tumor tissue was verified by immunohistochemical techniques staining for cleaved caspase-3 p11 subunit. The results demonstrated the potential of this imaging probe to guide the evaluation of chemotherapy treatment.

Paper Details

Date Published: 23 February 2012
PDF: 6 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 822306 (23 February 2012); doi: 10.1117/12.908943
Show Author Affiliations
Quihong Yang, The Univ. of Kansas (United States)
Huizhong Cui, The Univ. of Kansas (United States)
Shuang Cai, The Univ. of Kansas (United States)
Xinmai Yang, The Univ. of Kansas (United States)
M. Laird Forrest, The Univ. of Kansas (United States)


Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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