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Proceedings Paper

Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo
Author(s): Arie Krumholz; Grigory S. Filonov; Jun Xia; Junjie Yao; Vladislav V. Verkhusha; Lihong V. Wang
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Paper Abstract

Genetically encoded probes powerfully and non-invasively target specific tissues, cells, and subcellular locations. iRFP, a novel near-infrared fluorescent protein with low quantum yield whose absorption and fluorescence maxima are located at wavelengths longer than the Q-band of hemoglobin absorption, is ideal for PAT. Here, we report on an in vitro comparison of iRFP with other far-red fluorescent proteins, and its use in imaging a mouse tumor xenograft model. In an in vivo experiment, we stably transfected iRFP into MTLn3 adenocarcinoma cells and injected them into the mammary fat pad of female SCID/NCr mice, then imaged the resulting tumors two and three weeks post injection. The contrast increase from the protein expression was high enough to clearly separate the tumor region from the rest of the animal.

Paper Details

Date Published: 23 February 2012
PDF: 5 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82233A (23 February 2012); doi: 10.1117/12.908927
Show Author Affiliations
Arie Krumholz, Washington Univ. in St. Louis (United States)
Grigory S. Filonov, Albert Einstein College of Medicine (United States)
Jun Xia, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Vladislav V. Verkhusha, Albert Einstein College of Medicine (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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