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Proceedings Paper

Analysis of human aorta using fluorescence lifetime imaging microscopy (FLIM)
Author(s): Gislaine Vieira-Damiani; J. Adur; D. P. Ferro; R. L. Adam; V. Pelegati; A. Thomáz; C. L. Cesar; K. Metze
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Paper Abstract

The use of photonics has improved our understanding of biologic phenomena. For the study of the normal and pathologic architecture of the aorta the use of Two-Photon Excited Fluorescence (TPEF) and Second Harmonic Generation showed interesting details of morphologic changes of the elastin-collagen architecture during aging or development of hypertension in previous studies. In this investigation we tried to apply fluorescence lifetime imaging (FLIM) for the morphologic analysis of human aortas. The aim of our study was to use FLIM in non-stained formalin-fixed and paraffin-embedded samples of the aorta ascendants in hypertensive and normotensive patients of various ages, examining two different topographical regions. The FLIM-spectra of collagen and elastic fibers were clearly distinguishable, thus permitting an exact analysis of unstained material on the microscopic level. Moreover the FLIM spectrum of elastic fibers revealed variations between individual cases, which indicate modifications on a molecular level and might be related to FLIM age or diseases states and reflect modifications on a molecular level.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260S (9 February 2012); doi: 10.1117/12.908918
Show Author Affiliations
Gislaine Vieira-Damiani, Univ. Estadual de Campinas (Brazil)
J. Adur, Univ. Estadual de Campinas (Brazil)
National Univ. of Entre Rios (Argentina)
D. P. Ferro, Univ. Estadual de Campinas (Brazil)
R. L. Adam, National Univ. of Entre Rios (Argentina)
V. Pelegati, Univ. Estadual de Campinas (Brazil)
A. Thomáz, Univ. Estadual de Campinas (Brazil)
C. L. Cesar, Univ. Estadual de Campinas (Brazil)
K. Metze, Univ. Estadual de Campinas (Brazil)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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