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Proceedings Paper

Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization
Author(s): Hod Dana; Anat Marom; Nimrod Kruger; Aviv Ellman; Shy Shoham
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Paper Abstract

The development of rapid volumetric imaging systems for functional multiphoton microscopy is essential for dynamical imaging of large-scale neuronal network activity. Here, we introduce a line-illuminating temporal-focusing microscope capable of rapid three-dimensional imaging at 10-20 volumes/sec, and study the system's characteristics both theoretically and experimentally. We demonstrate that our system is capable of functional volumetric calcium imaging of distributed neuronal activity patterns, and introduce a computational strategy for activity reconstruction in strongly scattering media.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822603 (9 February 2012); doi: 10.1117/12.908844
Show Author Affiliations
Hod Dana, Technion-Israel Institute of Technology (Israel)
Anat Marom, Technion-Israel Institute of Technology (Israel)
Nimrod Kruger, Technion-Israel Institute of Technology (Israel)
Aviv Ellman, Technion-Israel Institute of Technology (Israel)
Shy Shoham, Technion-Israel Institute of Technology (Israel)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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