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Proceedings Paper

In vivo pump-probe microscopy of melanoma and pigmented lesions
Author(s): Jesse W. Wilson; Simone Degan; Tanya Mitropoulos; M. Angelica Selim; Jennifer Y. Zhang; Warren S. Warren
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Paper Abstract

A growing number of dermatologists and pathologists are concerned that the rapidly rising incidence of melanoma reflects not a true 'epidemic' but an increasing tendency to overdiagnose pigmented lesions. Addressing this problem requires both a better understanding of early-stage melanoma and new diagnostic criteria based on more than just cellular morphology and architecture. Here we present a method for in-vivo optical microscopy that utilizes pump-probe spectroscopy to image the distribution of the two forms of melanin in skin: eumelanin and pheomelanin. Images are acquired in a scanning microscope with a sensitive modulation transfer technique by analyzing back-scattered probe light with a lock-in amplifier. Early-stage melanoma is studied in a human skin xenografted mouse model. Individual melanocytes have been observed, in addition to pigmented keratinocytes. Combining the pump-probe images simultaneously with other noninvasive laser microscopy methods (confocal reflectance, multiphoton autofluorescence, and second harmonic generation) allows visualization of the skin architecture, framing the functional pump-probe image in the context of the surrounding tissue morphology. It is found that pump-probe images of melanin can be acquired with low peak intensities, enabling wide field-of-view pigmentation surveys. Finally, we investigate the diagnostic potential of the additional chemical information available from pump-probe microscopy.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822602 (9 February 2012); doi: 10.1117/12.908821
Show Author Affiliations
Jesse W. Wilson, Duke Univ. (United States)
Simone Degan, Duke Univ. (United States)
Tanya Mitropoulos, Duke Univ. (United States)
M. Angelica Selim, Duke Univ. (United States)
Jennifer Y. Zhang, Duke Univ. (United States)
Warren S. Warren, Duke Univ. (United States)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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