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Proceedings Paper

Combined optoacoustic and high frequency ultrasound imaging of live mouse embryos
Author(s): Parag V. Chitnis; Orlando Aristizbal; Erwan Filoux; Ashwinkumar Sampathkumar; Jonathan Mamou; Daniel H. Turnbull; Jeffrey A. Ketterling
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Paper Abstract

The cell differentiation and proliferation of the central nervous system (CNS) are closely related to vascular development. An imaging protocol that integrated optoacoustics (OA) with high-frequency ultrasound (HFU) was developed for in vivo imaging of brain ventricles and vasculature in mouse embryos. A 40-MHz, co-polymer, 5-element annular-array transducer with a geometric focus of 12 mm was modified to accommodate free-beam, coaxial illumination. Three-dimensional (3-D) data sets were acquired by raster scanning the transducer-optics assembly in 50-μm increments. A single intact conceptus from an anesthetized mouse was surgically exposed into PBS-filled Petri-dish. An 800-μm spot illumination from a pulsed, 532-nm, Nd-YAG laser was synchronized with a high-voltage impulse excitation of the central array element to facilitate simultaneous and spatially coregistered OA and HFU data acquisition. The resulting OA and HFU signals from each scan location were recorded on all five array channels and post-processed using a synthetic-focusing algorithm to enhance the depth of field (DOF). Dual-modality images were acquired from mouse embryos at E11.5, E12.5, and E13.5 days of gestation. The extended DOF allowed morphologically accurate visualization of the embryonic head. The brain ventricles were segmented from the HFU data and rendered in 3-D. The OA data provided visualization of the vascular plexus as well as individual blood vessels. Feasibility of spatially co-registered, low-cost dual-modality in vivo imaging of mouse embryos was demonstrated.

Paper Details

Date Published: 23 February 2012
PDF: 6 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 822314 (23 February 2012); doi: 10.1117/12.908716
Show Author Affiliations
Parag V. Chitnis, Riverside Research Institute (United States)
Orlando Aristizbal, New York Univ. School of Medicine (United States)
Erwan Filoux, Riverside Research Institute (United States)
Ashwinkumar Sampathkumar, Riverside Research Institute (United States)
Jonathan Mamou, Riverside Research Institute (United States)
Daniel H. Turnbull, New York Univ. School of Medicine (United States)
Jeffrey A. Ketterling, Riverside Research Institute (United States)


Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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