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Proceedings Paper

Melanin fluorescence spectra by step-wise three photon excitation
Author(s): Zhenhua Lai; Josef Kerimo; Charles A. DiMarzio
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Paper Abstract

Melanin is the characteristic chromophore of human skin with various potential biological functions. Kerimo discovered enhanced melanin fluorescence by stepwise three-photon excitation in 2011. In this article, step-wise three-photon excited fluorescence (STPEF) spectrum between 450 nm -700 nm of melanin is reported. The melanin STPEF spectrum exhibited an exponential increase with wavelength. However, there was a probability of about 33% that another kind of step-wise multi-photon excited fluorescence (SMPEF) that peaks at 525 nm, shown by previous research, could also be generated using the same process. Using an excitation source at 920 nm as opposed to 830 nm increased the potential for generating SMPEF peaks at 525 nm. The SMPEF spectrum peaks at 525 nm photo-bleached faster than STPEF spectrum.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82270J (9 February 2012); doi: 10.1117/12.908710
Show Author Affiliations
Zhenhua Lai, Northeastern Univ. (United States)
CenSSIS (United States)
Josef Kerimo, Northeastern Univ. (United States)
CenSSIS (United States)
Charles A. DiMarzio, Northeastern Univ. (United States)
CenSSIS (United States)


Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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