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Proceedings Paper

Raman spectroscopic imaging as complementary tool for histopathologic assessment of brain tumors
Author(s): Christoph Krafft; Norbert Bergner; Bernd Romeike; Rupert Reichart; Rolf Kalff; Kathrin Geiger; Matthias Kirsch; Gabriele Schackert; Jürgen Popp
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Paper Abstract

Raman spectroscopy enables label-free assessment of brain tissues and tumors based on their biochemical composition. Combination of the Raman spectra with the lateral information allows grading of tumors, determining the primary tumor of brain metastases and delineating tumor margins - even during surgery after coupling with fiber optic probes. This contribution presents exemplary Raman spectra and images collected from low grade and high grade regions of astrocytic gliomas and brain metastases. A region of interest in dried tissue sections encompassed slightly increased cell density. Spectral unmixing by vertex component analysis (VCA) and N-FINDR resolved cell nuclei in score plots and revealed the spectral contributions of nucleic acids, cholesterol, cholesterol ester and proteins in endmember signatures. The results correlated with the histopathological analysis after staining the specimens by hematoxylin and eosin. For a region of interest in non-dried, buffer immersed tissue sections image processing was not affected by drying artifacts such as denaturation of biomolecules and crystallization of cholesterol. Consequently, the results correspond better to in vivo situations. Raman spectroscopic imaging of a brain metastases from renal cell carcinoma showed an endmember with spectral contributions of glycogen which can be considered as a marker for this primary tumor.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82074I (9 February 2012); doi: 10.1117/12.908668
Show Author Affiliations
Christoph Krafft, Institute of Photonic Technology (Germany)
Norbert Bergner, Institute of Photonic Technology (Germany)
Bernd Romeike, Univ. Hospital Jena (Germany)
Rupert Reichart, Univ. Hospital Jena (Germany)
Rolf Kalff, Univ. Hospital Jena (Germany)
Kathrin Geiger, Univ. Hospital Dresden (Germany)
Matthias Kirsch, Univ. Hospital Dresden (Germany)
Gabriele Schackert, Univ. Hospital Dresden (Germany)
Jürgen Popp, Institute of Photonic Technology (Germany)
Friedrich-Schiller-Univ. Jena (Germany)


Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg; Melissa J. Suter; Brian Jet-Fei Wong; Justus F. Ilgner; Stephen Lam; Bodo E. Knudsen; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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