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Proceedings Paper

Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC
Author(s): Wolfgang Becker; Bertram Su; Axel Bergmann
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Paper Abstract

We present a technique that records transient effects in the fluorescence lifetime of a sample with spatial resolution along a one-dimensional scan. The technique is based on scanning a sample with a high-frequency pulsed laser beam, and building up a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses, and the time after a stimulation of the sample. The maximum resolution at which lifetime changes can be recorded is given by the line scan time. With repetitive stimulation and triggered accumulation transient lifetime effects can be resolved at a resolution of about one millisecond.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260C (9 February 2012); doi: 10.1117/12.908663
Show Author Affiliations
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Bertram Su, Becker & Hickl GmbH (Germany)
Axel Bergmann, Becker & Hickl GmbH (Germany)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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