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Proceedings Paper

Correction accuracy in image-based adaptive optics for nonlinear microscopy
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Paper Abstract

We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.

Paper Details

Date Published: 2 February 2012
PDF: 11 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 822709 (2 February 2012); doi: 10.1117/12.908647
Show Author Affiliations
Aurélie Facomprez, Ecole Polytechnique ParisTech, CNRS, INSERM (France)
Emmanuel Beaurepaire, Ecole Polytechnique ParisTech, CNRS, INSERM (France)
Delphine Débarre, Ecole Polytechnique ParisTech, CNRS, INSERM (France)


Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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