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Proceedings Paper

Imaging immune response of skin mast cells in vivo with two-photon microscopy
Author(s): Chunqiang Li; Riikka K. Pastila; Charles P. Lin
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Paper Abstract

Intravital multiphoton microscopy has provided insightful information of the dynamic process of immune cells in vivo. However, the use of exogenous labeling agents limits its applications. There is no method to perform functional imaging of mast cells, a population of innate tissue-resident immune cells. Mast cells are widely recognized as the effector cells in allergy. Recently their roles as immunoregulatory cells in certain innate and adaptive immune responses are being actively investigated. Here we report in vivo mouse skin mast cells imaging with two-photon microscopy using endogenous tryptophan as the fluorophore. We studied the following processes. 1) Mast cells degranulation, the first step in the mast cell activation process in which the granules are released into peripheral tissue to trigger downstream reactions. 2) Mast cell reconstitution, a procedure commonly used to study mast cells functioning by comparing the data from wild type mice, mast cell-deficient mice, and mast-cell deficient mice reconstituted with bone marrow-derived mast cells (BMMCs). Imaging the BMMCs engraftment in tissue reveals the mast cells development and the efficiency of BMMCs reconstitution. We observed the reconstitution process for 6 weeks in the ear skin of mast cell-deficient Kit wsh/ w-sh mice by two-photon imaging. Our finding is the first instance of imaging mast cells in vivo with endogenous contrast.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82070F (9 February 2012); doi: 10.1117/12.908540
Show Author Affiliations
Chunqiang Li, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
The Univ. of Texas at El Paso (United States)
Riikka K. Pastila, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)
Radiation and Nuclear Safety Authority (Finland)
Charles P. Lin, Wellman Ctr. for Photomedicine, Massachusetts General Hospital, Harvard Medical School (United States)


Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg; Melissa J. Suter; Brian Jet-Fei Wong; Justus F. Ilgner; Stephen Lam; Bodo E. Knudsen; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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