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Proceedings Paper

Multimodal full-field optical coherence tomography on biological tissue: toward all optical digital pathology
Author(s): F. Harms; E. Dalimier; P. Vermeulen; A. Fragola; A. C. Boccara
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Paper Abstract

Optical Coherence Tomography (OCT) is an efficient technique for in-depth optical biopsy of biological tissues, relying on interferometric selection of ballistic photons. Full-Field Optical Coherence Tomography (FF-OCT) is an alternative approach to Fourier-domain OCT (spectral or swept-source), allowing parallel acquisition of en-face optical sections. Using medium numerical aperture objective, it is possible to reach an isotropic resolution of about 1x1x1 ìm. After stitching a grid of acquired images, FF-OCT gives access to the architecture of the tissue, for both macroscopic and microscopic structures, in a non-invasive process, which makes the technique particularly suitable for applications in pathology. Here we report a multimodal approach to FF-OCT, combining two Full-Field techniques for collecting a backscattered endogeneous OCT image and a fluorescence exogeneous image in parallel. Considering pathological diagnosis of cancer, visualization of cell nuclei is of paramount importance. OCT images, even for the highest resolution, usually fail to identify individual nuclei due to the nature of the optical contrast used. We have built a multimodal optical microscope based on the combination of FF-OCT and Structured Illumination Microscopy (SIM). We used x30 immersion objectives, with a numerical aperture of 1.05, allowing for sub-micron transverse resolution. Fluorescent staining of nuclei was obtained using specific fluorescent dyes such as acridine orange. We present multimodal images of healthy and pathological skin tissue at various scales. This instrumental development paves the way for improvements of standard pathology procedures, as a faster, non sacrificial, operator independent digital optical method compared to frozen sections.

Paper Details

Date Published: 29 February 2012
PDF: 8 pages
Proc. SPIE 8216, Multimodal Biomedical Imaging VII, 821609 (29 February 2012); doi: 10.1117/12.908459
Show Author Affiliations
F. Harms, Ecole Supérieure de Physique et de Chimie Industrielles (France)
LLTECH SAS (France)
E. Dalimier, LLTECH SAS (France)
P. Vermeulen, Ecole Supérieure de Physique et de Chimie Industrielles (France)
A. Fragola, Ecole Supérieure de Physique et de Chimie Industrielles (France)
A. C. Boccara, Ecole Supérieure de Physique et de Chimie Industrielles (France)
LLTECH SAS (France)


Published in SPIE Proceedings Vol. 8216:
Multimodal Biomedical Imaging VII
Fred S. Azar; Xavier Intes, Editor(s)

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