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Proceedings Paper

Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy
Author(s): Honghai Liu; Wan Qin; Yonghong Shao; Qiuying Liu; Zhen Ma; Thomas K. Borg; Bruce Z. Gao
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Paper Abstract

Utilizing a custom-built, on-stage incubator-combined, two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging system, we observed new-sarcomere addition in rat neonatal cardiomyocytes during 10 hours of on-stage incubation. This addition occurred at one end of an existing myofibril, the sides of existing myofibrils, and at the interstice of several separated myofibrils; in the cases of the latter two, we observed mature myofibrils acting as templates. We found that during sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric length during maturation.

Paper Details

Date Published: 23 February 2012
PDF: 7 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 822524 (23 February 2012); doi: 10.1117/12.908381
Show Author Affiliations
Honghai Liu, Clemson Univ. (United States)
Wan Qin, Clemson Univ. (United States)
Yonghong Shao, Shenzhen Univ. (China)
Qiuying Liu, Clemson Univ. (United States)
Zhen Ma, Clemson Univ. (United States)
Thomas K. Borg, Medical Univ. of South Carolina (United States)
Bruce Z. Gao, Clemson Univ. (United States)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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