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Proceedings Paper

Full-field OCT combined with optical tweezer
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Paper Abstract

We present an optical tweezer technique assisted full-field optical coherence tomography (FF-OCT) system. The proposed scheme enables ultrahigh-resolution OCT imaging of a floating object optically trapped by single-beam gradient force in medium. The set up consists of a Linnik type of white light interference microscope combined with an optical tweezer system. The optical trap is formed by tightly focusing a 1064 nm Q-switching pulsed laser beam with a microscope objective lens of high numerical aperture (1.0 NA) in sample arm of the OCT interferometer. This co-sharing of probe channel between two of systems enables concurrent actions of trapping and OCT imaging for the sample. OCT imaging of the sample in depth can achieve by positioning the coherence gating with displacement of reference arm in the OCT interferometer. To demonstrate the efficacy of the system, micron-sized dielectric particles and living cells in solution are simultaneously trapped and optically sliced with cellular resolution.

Paper Details

Date Published: 3 February 2012
PDF: 6 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271J (3 February 2012); doi: 10.1117/12.908380
Show Author Affiliations
Woo June Choi, Gwangju Institute of Science and Technology (Korea, Republic of)
Kwan Seob Park, Gwangju Institute of Science and Technology (Korea, Republic of)
Tae Joong Eom, Gwangju Institute of Science and Technology (Korea, Republic of)
Myung-Kyu Oh, Gwangju Institute of Science and Technology (Korea, Republic of)
Byeong-Ha Lee, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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