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Proceedings Paper

Raman spectroscopic analysis of human tissue engineered oral mucosa constructs (EVPOME) perturbed by physical and biochemical methods
Author(s): Alexander Khmaladze; Arindam Ganguly; Mekhala Raghavan; Shiuhyang Kuo; Jacqueline H. Cole; Cynthia L. Marcelo; Stephen E. Feinberg; Kenji Izumi; Michael D. Morris
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Paper Abstract

We show the application of near-infrared Raman Spectroscopy to in-vitro monitoring of the viability of tissue constructs (EVPOMEs). During their two week production period EVPOME may encounter thermal, chemical or biochemical stresses that could cause development to cease, rendering the affected constructs useless. We discuss the development of a Raman spectroscopic technique to study EVPOMEs noninvasively, with the ultimate goal of applying it in-vivo. We identify Raman spectroscopic failure indicators for EVPOMEs, which are stressed by temperature, and discuss the implications of varying calcium concentration and pre-treatment of the human keratinocytes with Rapamycin. In particular, Raman spectra show correlation of the peak height ratios of CH2 deformation to phenylalanine ring breathing, providing a Raman metric to distinguish between viable and nonviable constructs. We also show the results of singular value decomposition analysis, demonstrating the applicability of Raman spectroscopic technique to both distinguish between stressed and non-stressed EVPOME constructs, as well as between EVPOMEs and bare AlloDerm® substrates, on which the oral keratinocytes have been cultured. We also discuss complications arising from non-uniform thickness of the AlloDerm® substrate and the cultured constructs, as well as sampling protocols used to detect local stress and other problems that may be encountered in the constructs.

Paper Details

Date Published: 31 January 2012
PDF: 6 pages
Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 82190J (31 January 2012); doi: 10.1117/12.908281
Show Author Affiliations
Alexander Khmaladze, Univ. of Michigan, Ann Arbor (United States)
Arindam Ganguly, Univ. of Michigan, Ann Arbor (United States)
Mekhala Raghavan, Univ. of Michigan, Ann Arbor (United States)
Shiuhyang Kuo, Univ. of Michigan, Ann Arbor (United States)
Jacqueline H. Cole, Univ. of Michigan, Ann Arbor (United States)
Cynthia L. Marcelo, Univ. of Michigan, Ann Arbor (United States)
Stephen E. Feinberg, Univ. of Michigan, Ann Arbor (United States)
Kenji Izumi, Niigata Univ. (Japan)
Michael D. Morris, Univ. of Michigan, Ann Arbor (United States)


Published in SPIE Proceedings Vol. 8219:
Biomedical Vibrational Spectroscopy V: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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