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Proceedings Paper

Cell morphology classification in phase contrast microscopy image reducing halo artifact
Author(s): Mi-Sun Kang; Soo-Min Song; Hana Lee; Myoung-Hee Kim
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Paper Abstract

Since the morphology of tumor cells is a good indicator of their invasiveness, we used time-lapse phase-contrast microscopy to examine the morphology of tumor cells. This technique enables long-term observation of the activity of live cells without photobleaching and phototoxicity which is common in other fluorescence-labeled microscopy. However, it does have certain drawbacks in terms of imaging. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary. In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we examined cell morphology to classify tumor cells as malignant and benign.

Paper Details

Date Published: 3 February 2012
PDF: 9 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271I (3 February 2012); doi: 10.1117/12.908070
Show Author Affiliations
Mi-Sun Kang, Ewha Womans Univ. (Korea, Republic of)
Soo-Min Song, Ewha Womans Univ. (Korea, Republic of)
Hana Lee, Ewha Womans Univ. (Korea, Republic of)
Myoung-Hee Kim, Ewha Womans Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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