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Proceedings Paper

Watching stem cells at work with a flexible multiphoton tomograph
Author(s): Aisada Uchugonova; Robert Hoffmann; Martin Weinigel; Karsten König
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Paper Abstract

There is a high demand for non-invasive imaging techniques that allow observation of stem cells in their native environment without significant input on cell metabolism, reproduction, and behavior. Easy accessible hair follicle pluripotent stem cells in the bulge area and dermal papilla are potential sources for stem cell based therapy. It has been shown that these cells are able to generate hair, non-follicle skin cells, nerves, vessels, smooth muscles etc. and may participate in wound healing processes. We report on the finding of nestin-GFP expressing stem cells in their native niche in the bulge of the hair follicle of living mice by using high-resolution in-vivo multiphoton tomography. The 3D imaging with submicron resolution was based on two-photon induced fluorescence and second harmonic generation (SHG) of collagen. Migrating stem cells from the bulge to their microenvironment have been detected inside the skin during optical deep tissue sectioning.

Paper Details

Date Published: 15 February 2012
PDF: 8 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260V (15 February 2012); doi: 10.1117/12.908027
Show Author Affiliations
Aisada Uchugonova, Saarland Univ. (Germany)
Univ. of California, San Diego (United States)
AntiCancer Inc. (United States)
Robert Hoffmann, Univ. of California, San Diego (United States)
Anticancer Inc. (United States)
Martin Weinigel, JenLab GmbH (Germany)
Karsten König, Saarland Univ. (Germany)
JenLab GmbH (Germany)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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