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Proceedings Paper

Surface modification of upconversion nanoparticles with amphiphilic chitosan for cancer cell imaging
Author(s): Sisi Cui; Hongyan Zhu; Haiyan Chen; Junmei Tian; Wei R. Chen; Yueqing Gu
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Paper Abstract

Upconversion nanoparticles (UCNPs) as a new kind of biological luminescence materials have many advantages comparing with organic fluorescence probes and semi-conductive quantum dots, such as sharp fluorescence emission, long emission lifetimes, high optical and chemical stability and low toxicity, especially low auto-fluorescence background and deep tissue penetration under near-infrared (NIR) light excitation for bioimaging. Herein, we demonstrate a facile approach to transfer UCNPs from hydrophobic to hydrophilic and the use of these UCNPs for cell imaging. Oleic acid-capped UCNPs based on NaYF4 were synthesized and modified with amphiphilic chitosan derivative through hydrophobic interaction. The as-prepared chitosan coated UCNPs with an average diameter of 35 nm were mono-dispersed in aqueous solution and possess good optical properties upon NIR light excitation. Cell viability assays indicated the low cytotoxicity and good biocompatibility of chitosan-coated UCNPs. Cell imaging results demonstrated that chitosan-coated UCNPs had great potential for bioimaing and biolabeling. Our work suggests a feasible method to modify OA-UCNPs with amphiphilic polymer and the promise of chitosan-based UCNPs for bioimaging application.

Paper Details

Date Published: 14 February 2012
PDF: 9 pages
Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240V (14 February 2012); doi: 10.1117/12.907916
Show Author Affiliations
Sisi Cui, China Pharmaceutical Univ. (China)
Hongyan Zhu, China Pharmaceutical Univ. (China)
Haiyan Chen, China Pharmaceutical Univ. (China)
Junmei Tian, China Pharmaceutical Univ. (China)
Wei R. Chen, Univ. of Central Oklahoma (United States)
Yueqing Gu, China Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 8224:
Biophotonics and Immune Responses VII
Wei R. Chen, Editor(s)

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