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Proceedings Paper

Two-photon imaging and spectroscopy of fresh human colon biopsies
Author(s): R. Cicchi; A. Sturiale; G. Nesi; F. Tonelli; F. S. Pavone
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Paper Abstract

Two-photon fluorescence (TPEF) microscopy is a powerful tool to image human tissues up to 200 microns depth without any exogenously added probe. TPEF can take advantage of the autofluorescence of molecules intrinsically contained in a biological tissue, as such NADH, elastin, collagen, and flavins. Two-photon microscopy has been already successfully used to image several types of tissues, including skin, muscles, tendons, bladder. Nevertheless, its usefulness in imaging colon tissue has not been deeply investigated yet. In this work we have used combined two-photon excited fluorescence (TPEF), second harmonic generation microscopy (SHG), fluorescence lifetime imaging microscopy (FLIM), and multispectral two-photon emission detection (MTPE) to investigate different kinds of human ex-vivo fresh biopsies of colon. Morphological and spectroscopic analyses allowed to characterize both healthy mucosa, polyp, and colon samples in a good agreement with common routine histology. Even if further analysis, as well as a more significant statistics on a large number of samples would be helpful to discriminate between low, mild, and high grade cancer, our method is a promising tool to be used as diagnostic confirmation of histological results, as well as a diagnostic tool in a multiphoton endoscope or colonoscope to be used in in-vivo imaging applications.

Paper Details

Date Published: 14 February 2012
PDF: 7 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263S (14 February 2012); doi: 10.1117/12.907822
Show Author Affiliations
R. Cicchi, European Lab. for Non-linear Spectroscopy, Univ. of Florence (Italy)
A. Sturiale, Univ. of Florence (Italy)
G. Nesi, Univ. of Florence (Italy)
F. Tonelli, Univ. of Florence (Italy)
F. S. Pavone, European Lab. for Non-linear Spectroscopy, Univ. of Florence (Italy)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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