Share Email Print
cover

Proceedings Paper

Spectrally resolved visualization of fluorescent dyes permeating into skin
Author(s): Ulf Maeder; Thorsten Bergmann; Sebastian Beer; Jan Michael Burg; Thomas Schmidts; Frank Runkel; Martin Fiebich
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We present a spectrally resolved confocal imaging approach to qualitatively asses the overall uptake and the penetration depth of fluorescent dyes into biological tissue. We use a confocal microscope with a spectral resolution of 5 nm to measure porcine skin tissue after performing a Franz-Diffusion experiment with a submicron emulsion enriched with the fluorescent dye Nile Red. The evaluation uses linear unmixing of the dye and the tissue autofluorescence spectra. The results are combined with a manual segmentation of the skin's epidermis and dermis layers to assess the penetration behavior additionally to the overall uptake. The diffusion experiments, performed for 3h and 24h, show a 3-fold increased dye uptake in the epidermis and dermis for the 24h samples. As the method is based on spectral information it does not face the problem of superimposed dye and tissue spectra and therefore is more precise compared to intensity based evaluation methods.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 822523 (9 February 2012); doi: 10.1117/12.907815
Show Author Affiliations
Ulf Maeder, Technische Hochschule Mittelhessen (Germany)
Thorsten Bergmann, Technische Hochschule Mittelhessen (Germany)
Sebastian Beer, Technische Hochschule Mittelhessen (Germany)
Jan Michael Burg, Technische Hochschule Mittelhessen (Germany)
Thomas Schmidts, Technische Hochschule Mittelhessen (Germany)
Frank Runkel, Technische Hochschule Mittelhessen (Germany)
Martin Fiebich, Technische Hochschule Mittelhessen (Germany)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

© SPIE. Terms of Use
Back to Top