Share Email Print

Proceedings Paper

Multiphoton fluorescence spectra and lifetimes of biliverdins and their protein-associated complex
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

To investigate whether endogenous biliverdins can serve as a fluorescence metabolic marker in cancer diagnosis, we measured their multiphoton fluorescence spectra and lifetimes with femtosecond Cr:forsterite laser. Excited at 1230nm, the two-photon fluorescence of biliverdins peaks around 670nm. The corresponding lifetime (<100ps) was much shorter than those of porphyrins (~10ns), which is another commonly present metabolites in living cells. Further mixing biliverdins with proteins like fetal bovine serum (FBS), biliverdins reductase A (BVRA), or heme oxygenase-1 (HO-1), the yields of red autofluorescences didn't change a lot, but the corresponding lifetimes with HO-1 and BSA were lengthened to 200~300ps. This indicates that biliverdin can have an association with these proteins and change its lifetime. These spectral and temporal characteristics of fluorescence make biliverdin a potential marker fluorophore for hyperspectral diagnosis on the heme catabolism in human cells or tissues.

Paper Details

Date Published: 2 February 2012
PDF: 5 pages
Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82330P (2 February 2012); doi: 10.1117/12.907725
Show Author Affiliations
Chin-Jie Huang, National Taiwan Univ. (Taiwan)
Cheng-Ham Wu, National Taiwan Univ. (Taiwan)
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8233:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top