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Proceedings Paper

Optical modulation of astrocyte network using ultrashort pulsed laser
Author(s): Jonghee Yoon; Taeyun Ku; Kyuha Chong; Seung-Wook Ryu; Chulhee Choi
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Paper Abstract

Astrocyte, the most abundant cell type in the central nervous system, has been one of major topics in neuroscience. Even though many tools have been developed for the analysis of astrocyte function, there has been no adequate tool that can modulates astrocyte network without pharmaceutical or genetic interventions. Here we found that ultrashort pulsed laser stimulation can induce label-free activation of astrocytes as well as apoptotic-like cell death in a dose-dependent manner. Upon irradiation with high intensity pulsed lasers, the irradiated cells with short exposure time showed very rapid mitochondria fragmentation, membrane blebbing and cytoskeletal retraction. We applied this technique to investigate in vivo function of astrocyte network in the CNS: in the aspect of neurovascular coupling and blood-brain barrier. We propose that this noninvasive technique can be widely applied for in vivo study of complex cellular network.

Paper Details

Date Published: 9 February 2012
PDF: 5 pages
Proc. SPIE 8221, Optical Interactions with Tissue and Cells XXIII, 82210A (9 February 2012); doi: 10.1117/12.907692
Show Author Affiliations
Jonghee Yoon, KAIST (Korea, Republic of)
Taeyun Ku, KAIST (Korea, Republic of)
Kyuha Chong, KAIST (Korea, Republic of)
Seung-Wook Ryu, KAIST (Korea, Republic of)
Chulhee Choi, KAIST (Korea, Republic of)

Published in SPIE Proceedings Vol. 8221:
Optical Interactions with Tissue and Cells XXIII
E. Duco Jansen; Robert J. Thomas, Editor(s)

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