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Proceedings Paper

Identifying mitosis deep in tissue using dynamic light scattering fluctuation spectroscopy
Author(s): Ran An; Kwan Jeong; John Turek; David Nolte
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Paper Abstract

In the cell cycle, mitosis is the most dramatic phase, especially in Telophase and Cytokinesis. For single cells and cell monolayer, there are precise microscopic studies of mitosis, while for 3-D tissue such as tumor spheroids the light signal is obscured by the high background of diffusely scattered light. Therefore, the mitosis phase cannot be detected deep inside 3-D tissue using conventional microscopic techniques. In this work, we detect mitosis in living tissue using Tissue Dynamic Imaging (TDI). We trace depth-gated dynamic speckles from a tumor spheroid (up to 1mm in diameter) using coherence-gated digital holography imaging. Frequency-versus-time spectrograms depend on specific types of perturbation such as cell shape change, membrane undulation and cell organelles movements. By using these spectral responses as functional finger prints, we can identify mitosis events from different voxels at a specified depth inside tumor spheroids. By performing B-scans of the tumor spheroid, we generate 3-D mitosis maps (or movies) for the entire tumor spheroids. We show that for healthy tumor spheroids, the mitosis events only happen within the proliferating shell. We also compare results when anti-cancer drugs are applied to arrest, release and synchronize mitosis. This shows the application of TDI for drug screening. The technique can identify and monitor complex motilities inside 3-D tissue with a strong potential for drug diagnosis and developmental biology studies.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8222, Dynamics and Fluctuations in Biomedical Photonics IX, 82220G (9 February 2012); doi: 10.1117/12.907654
Show Author Affiliations
Ran An, Purdue Univ. (United States)
Kwan Jeong, Korean Military Institute (Korea, Republic of)
John Turek, Purdue Univ. (United States)
David Nolte, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 8222:
Dynamics and Fluctuations in Biomedical Photonics IX
Valery V. Tuchin; Donald D. Duncan; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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