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Proceedings Paper

Long-distance fluorescence lifetime imaging with stimulated emission and electronic time delay
Author(s): Po-Yen Lin; Shin-Shian Lee; Chia-Seng Chang; Fu-Jen Kao
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Paper Abstract

In this work, long-distance fluorescence lifetime imaging is realized by stimulated emission and electronic time delay control. Spatial coherence, as a result of stimulated emission, provides unattenuated fluorescence detection at long distance with low NA optics. The electronic trigger also provides a feasible way to change the pulse separation and probe the fluorescence lifetime in nano-second range. The lifetime of fluorophores is determined by measuring the stimulated emission signal at various the time delays between excitation and stimulation pulses. The characteristics of stimulated emission are investigated and the saturation condition is also presented.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82262T (9 February 2012); doi: 10.1117/12.907632
Show Author Affiliations
Po-Yen Lin, National Yang-Ming Univ. (Taiwan)
Academia Sinica (Taiwan)
Shin-Shian Lee, National Yang-Ming Univ. (Taiwan)
Chia-Seng Chang, Academia Sinica (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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