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Proceedings Paper

Application of second-harmonic generation microscopy for in vivo observation of structural change in human dermal collagen fiber caused by aging and/or UV exposure
Author(s): T. Yasui; M. Yonetsu; R. Tanaka; S. Fukushima; T. Yamashita; Y. Ogura; T. Hirao; T. Araki
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Paper Abstract

Second-harmonic-generation (SHG) microscopy is useful to visualize collagen fiber in biological tissues in vivo. In this paper, we applied our SHG microscopy equipped with a Cr:Forsterite laser to visualize human dermal collagen fiber in vivo. The obtained SHG images indicated the structural difference of dermal collagen fiber between different ages, for example, fine collagen fiber is densely distributed in 20's subjects whereas only thick collagen fiber is remained in 60's subjects. These results reflect structural change of collagen fiber caused by natural aging and/or photoaging. The SHG microscopy has a potential to become an in vivo collagen-sensitive microscopy for assessment of skin aging.

Paper Details

Date Published: 13 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263E (13 February 2012); doi: 10.1117/12.907596
Show Author Affiliations
T. Yasui, Univ. of Tokushima (Japan)
Osaka Univ. (Japan)
M. Yonetsu, Osaka Univ. (Japan)
R. Tanaka, Osaka Univ. (Japan)
S. Fukushima, Osaka Univ. (Japan)
T. Yamashita, Shiseido Co. (Japan)
Y. Ogura, Shiseido Co. (Japan)
T. Hirao, Shiseido Co. (Japan)
T. Araki, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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