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Proceedings Paper

Synthesis of dimeric cyclic RGD based near-infrared probe for in vivo tumor diagnosis
Author(s): Jie Cao; Shunan Wan; Junmei Tian; Xuemei Chi; Changli Du; Dawei Deng; Wei R. Chen; Yueqing Gu
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Paper Abstract

Cell adhesion molecule integrin αvβ3 is an excellent target for tumor interventions because of its unique expression on the surface of several types of solid tumor cells and on almost all sprouting tumor vasculatures. In this manuscript, we describe the synthesis of near-infrared (NIR) fluorochrome ICG-Der-02-labeled dimeric cyclic RGD peptides (ICG-Der-02-c(RGDyK)2) for in vivo tumor integrin targeting. The optical properties and structure of the probe were intensively characterized. Afterwards, the integrin specificity of the fluorescent probe was tested in vitro for receptor binding assay and fluorescence microscopy and in vivo for subcutaneous MDA-MB-231 and U87MG tumor targeting. The results indicated that after labeling RGD peptide, the optical properties of ICG-Der-02 showed no obvious change. Besides, in vitro and in vivo tumor targeting experiment indicated that the ICG-Der-02-c(RGDyK)2 probe with high integrin affinity showed excellent tumor activity accumulation. Noninvasive NIR fluorescence imaging is able to detect tumor integrin expression based upon the highly potent RGD peptide probe.

Paper Details

Date Published: 15 February 2012
PDF: 12 pages
Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240T (15 February 2012); doi: 10.1117/12.907567
Show Author Affiliations
Jie Cao, China Pharmaceutical Univ. (China)
Shunan Wan, China Pharmaceutical Univ. (China)
Junmei Tian, China Pharmaceutical Univ. (China)
Xuemei Chi, China Pharmaceutical Univ. (China)
Changli Du, China Pharmaceutical Univ. (China)
Dawei Deng, China Pharmaceutical Univ. (China)
Wei R. Chen, Univ. of Central Oklahoma (United States)
Yueqing Gu, China Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 8224:
Biophotonics and Immune Responses VII
Wei R. Chen, Editor(s)

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