Share Email Print
cover

Proceedings Paper

Multiphoton imaging with compact semiconductor disk lasers
Author(s): Pablo Loza-Alvarez; Rodrigo Aviles-Espinosa; David Artigas-García; Craig Hamilton; Graeme Malcolm
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We present the performance of a compact, non expensive, easy to use ultrafast semiconductor disk laser (1W average power, 1.5 ps, 500MHz) for multiphoton imaging. The laser's operating wavelengths of 970 nm makes it ideal for nonlinear excitation of GFP as it has a two-photon action cross section peak at this wavelength. This property relaxes the required peak powers for TPEF imaging. We show the suitability of this laser for in-vivo imaging with GFP and other dyes; at different penetration depths; time-lapse studies and SHG imaging. The laser performance is evaluated in commercial microscopes and in comparison with Ti:sapphire lasers.

Paper Details

Date Published: 14 February 2012
PDF: 10 pages
Proc. SPIE 8242, Vertical External Cavity Surface Emitting Lasers (VECSELs) II, 82420O (14 February 2012); doi: 10.1117/12.907456
Show Author Affiliations
Pablo Loza-Alvarez, ICFO - Institut de Ciències Fotòniques (Spain)
Rodrigo Aviles-Espinosa, ICFO - Institut de Ciències Fotòniques (Spain)
David Artigas-García, ICFO - Institut de Ciències Fotòniques (Spain)
Univ. Politècnica de Catalunya (Spain)
Craig Hamilton, M Squared Lasers LTD (United Kingdom)
Graeme Malcolm, M Squared Lasers LTD (United Kingdom)


Published in SPIE Proceedings Vol. 8242:
Vertical External Cavity Surface Emitting Lasers (VECSELs) II
Anne C. Tropper, Editor(s)

© SPIE. Terms of Use
Back to Top