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Proceedings Paper

Single optical fiber probe for optogenetics
Author(s): Ryan Falk; Mohammad Habibi; Ramin Pashaie
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Paper Abstract

With the advent of optogenetics, all optical control and visualization of the activity of specific cell types is possible. We have developed a fiber optic based probe to control/visualize neuronal activity deep in the brain of awake behaving animals. In this design a thin multimode optical fiber serves as the head of the probe to be inserted into the brain. This fiber is used to deliver excitation/stimulation optical pulses and guide a sample of the emission signal back to a detector. The major trade off in the design of such a system is to decrease the size of the fiber and intensity of input light to minimize physical damage and to avoid photobleaching/phototoxicity but to keep the S/N reasonably high. Here the excitation light, and the associated emission signal, are frequency modulated. Then the output of the detector is passed through a time-lens which compresses the distributed energy of the emission signal and maximizes the instantaneous S/N. By measuring the statistics of the noise, the structure of the time lens can be designed to achieve the global optimum of S/N. Theoretically, the temporal resolution of the system is only limited by the time lens diffraction limit. By adding a second detector, we eliminated the effect of input light fluctuations, imperfection of the optical filters, and back-reflection of the excitation light. We have also designed fibers and micro mechanical assemblies for distributed delivery and detection of light.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251Z (9 February 2012); doi: 10.1117/12.907453
Show Author Affiliations
Ryan Falk, Univ. of Wisconsin-Milwaukee (United States)
Mohammad Habibi, Univ. of Wisconsin-Milwaukee (United States)
Ramin Pashaie, Univ. of Wisconsin-Milwaukee (United States)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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