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Proceedings Paper

Spectral and lifetime domain measurements of rat brain tumours
Author(s): D. Abi Haidar; B. Leh; K. Allaoua; A. Genoux; R. Siebert; M. Steffenhagen; D. Peyrot; N. Sandeau; C. Vever-Bizet; G. Bourg-Heckly; I. Chebbi; M. Collado-Hilly
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Paper Abstract

During glioblastoma surgery, delineation of the brain tumour margins remains difficult especially since infiltrated and normal tissues have the same visual appearance. This problematic constitutes our research interest. We developed a fibre-optical fluorescence probe for spectroscopic and time domain measurements. First measurements of endogenous tissue fluorescence were performed on fresh and fixed rat tumour brain slices. Spectral characteristics, fluorescence redox ratios and fluorescence lifetime measurements were analysed. Fluorescence information collected from both, lifetime and spectroscopic experiments, appeared promising for tumour tissue discrimination. Two photon measurements were performed on the same fixed tissue. Different wavelengths are used to acquire two-photon excitation-fluorescence of tumorous and healthy sites.

Paper Details

Date Published: 9 February 2012
PDF: 11 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82074P (9 February 2012); doi: 10.1117/12.907411
Show Author Affiliations
D. Abi Haidar, Institut National de Physique Nucléaire et de Physique des Particules (France)
B. Leh, Lab. Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS, Univ. Paris 7 (France)
K. Allaoua, Lab. Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS, Univ. Paris 7 (France)
A. Genoux, Lab. Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS, Univ. Paris 7 (France)
R. Siebert, Lab. Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS, Univ. Paris 7 (France)
M. Steffenhagen, ANBioPhy, CNRS, Univ. Pierre et Marie Curie (France)
D. Peyrot, ANBioPhy, CNRS, Univ. Pierre et Marie Curie (France)
N. Sandeau, ANBioPhy, CNRS, Univ. Pierre et Marie Curie (France)
C. Vever-Bizet, ANBioPhy, CNRS, Univ. Pierre et Marie Curie (France)
G. Bourg-Heckly, ANBioPhy, CNRS, Univ. Pierre et Marie Curie (France)
I. Chebbi, SARL Nanobactérie, CNRS, Univ. Pierre et Marie Curie (France)
M. Collado-Hilly, INSERM (France)


Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; E. Duco Jansen; Andreas Mandelis; Kenton W. Gregory; Guillermo J. Tearney; Laura Marcu; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Melissa J. Suter; Stephen Lam; Matthew Brenner; Hyun Wook Kang; Bodo E. Knudsen; Henry Hirschberg; Steen Madsen; Brian Jet-Fei Wong; Justus F. Ilgner; Krzysztof Izdebski, Editor(s)

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