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Proceedings Paper

Buccal microbiology analyzed by infrared spectroscopy
Author(s): Geraldo Magno Alves de Abreu; Gislene Rodrigues da Silva; Sônia Khouri; Priscila Pereira Favero; Leandro Raniero; Airton Abrahão Martin
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Paper Abstract

Rapid microbiological identification and characterization are very important in dentistry and medicine. In addition to dental diseases, pathogens are directly linked to cases of endocarditis, premature delivery, low birth weight, and loss of organ transplants. Fourier Transform Infrared Spectroscopy (FTIR) was used to analyze oral pathogens Aggregatibacter actinomycetemcomitans ATCC 29523, Aggregatibacter actinomycetemcomitans-JP2, and Aggregatibacter actinomycetemcomitans which was clinically isolated from the human blood-CI. Significant spectra differences were found among each organism allowing the identification and characterization of each bacterial species. Vibrational modes in the regions of 3500-2800 cm-1, the 1484-1420 cm-1, and 1000-750 cm-1 were used in this differentiation. The identification and classification of each strain were performed by cluster analysis achieving 100% separation of strains. This study demonstrated that FTIR can be used to decrease the identification time, compared to the traditional methods, of fastidious buccal microorganisms associated with the etiology of the manifestation of periodontitis.

Paper Details

Date Published: 31 January 2012
PDF: 6 pages
Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 821912 (31 January 2012); doi: 10.1117/12.907398
Show Author Affiliations
Geraldo Magno Alves de Abreu, Univ. do Vale do Paraíba (Brazil)
Gislene Rodrigues da Silva, Univ. do Vale do Paraíba (Brazil)
Sônia Khouri, Univ. do Vale do Paraíba (Brazil)
Priscila Pereira Favero, Univ. do Vale do Paraíba (Brazil)
Leandro Raniero, Univ. do Vale do Paraíba (Brazil)
Airton Abrahão Martin, Univ. do Vale do Paraíba (Brazil)

Published in SPIE Proceedings Vol. 8219:
Biomedical Vibrational Spectroscopy V: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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