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Proceedings Paper

Fluorescent diamond nanoparticle as a probe of intracellular traffic in primary neurons in culture
Author(s): Xuan Loc Le; Aude-Marie Lepagnol-Bestel; Marie-Pierre Adam; Alice Thomas; Géraldine Dantelle; Cheng-Chun Chang; Nitin Mohan; Huan-Cheng Chang; François Treussart; Michel Simonneau
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Paper Abstract

Neurons display dendritic spines plasticity and morphology anomalies in numerous psychiatric and neurodegenerative diseases. These changes are associated to abnormal dendritic traffic that can be evidenced by fluorescence microscopy. As a fluorescent probe we propose to use fluorescent diamond nanoparticles with size of < 50 nm. Color centers embedded inside the diamond nanoparticles are perfectly photostable emitters allowing for long-term tracking. Nanodiamond carbon surface is also well suited for biomolecule functionalization to target specific cellular compartments. We show that fluorescent nanodiamonds can be spontaneously internalized in neurons in culture and imaged by confocal and Total Internal Reflection (TIRF) microscopy with a high signal over background ratio.

Paper Details

Date Published: 2 February 2012
PDF: 6 pages
Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 823203 (2 February 2012); doi: 10.1117/12.907298
Show Author Affiliations
Xuan Loc Le, CNRS, Ecole Normale Supérieure de Cachan (France)
Aude-Marie Lepagnol-Bestel, Ctr. de Psychiatrie et Neurosciences, INSERM, Univ. Paris Descartes (France)
Ctr. National de Génotypage (France)
Marie-Pierre Adam, CNRS, Ecole Normale Supérieure de Cachan (France)
Alice Thomas, Ctr. de Psychiatrie et Neurosciences, INSERM, Univ. Paris Descartes (France)
Géraldine Dantelle, CNRS, Ecole Polytechnique (France)
Cheng-Chun Chang, Institute of Atomic and Molecular Sciences (Taiwan)
National Taiwan Univ. (Taiwan)
Nitin Mohan, Institute of Atomic and Molecular Sciences (Taiwan)
National Taiwan Univ. (Taiwan)
Huan-Cheng Chang, Institute of Atomic and Molecular Sciences (Taiwan)
National Taiwan Univ. (Taiwan)
François Treussart, CNRS, Ecole Normale Supérieure de Cachan (France)
Michel Simonneau, Ctr. de Psychiatrie et Neurosciences, INSERM, Univ. Paris Descartes (France)


Published in SPIE Proceedings Vol. 8232:
Colloidal Nanocrystals for Biomedical Applications VII
Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski, Editor(s)

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