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Proceedings Paper

Effect of femtosecond laser radiation on morphofunctional state of neoplasm in vitro
Author(s): Tatyana Gening; Alexey Sysoliatin; Dinara Arslanova; Tatyana Abakumova; Vyacheslav Svetukhin; Inna Antoneeva
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Paper Abstract

The effect of femtosecond laser radiation provided by the Erbium fiber laser with the pulse duration of 10-13 s, peak and average power of 6,0±0,3 kW and 1,26±0,15 mW, respectively, wavelength λ of 1,55 μm has been studied in the experiments on rat ascitic ovarian tumor cell in vitro. Irradiation has been performed at the average intensity of 0,033±0.002 mW/cm2 at two expositions under femtosecond laser radiation of 600 and 900 s. The membrane topology and rigidity of the cancer cells have been estimated with the Scanning probe microscopy (SolverPro, NT-MDT, Russia). Besides, the viability and apoptosis of the cancer cells have been estimated. Free-radical processes and antioxidant enzyme activity have been studied in cancer cell lysate. Femtosecond laser irradiation was established to increases the activity of the "Lipoperoxidation - antioxidants" system in neoplasm, enhance the apoptosis, decrease the viability, and change cancer cell membrane topology and rigidity in vitro depending on the energy density of the irradiation.

Paper Details

Date Published: 9 February 2012
PDF: 10 pages
Proc. SPIE 8221, Optical Interactions with Tissue and Cells XXIII, 82210B (9 February 2012); doi: 10.1117/12.907271
Show Author Affiliations
Tatyana Gening, Ulyanovsk State Univ. (Russian Federation)
Alexey Sysoliatin, A. M. Prokhorov General Physics Institute (Russian Federation)
Dinara Arslanova, Ulyanovsk State Univ. (Russian Federation)
Tatyana Abakumova, Ulyanovsk State Univ. (Russian Federation)
Vyacheslav Svetukhin, Ulyanovsk State Univ. (Russian Federation)
Inna Antoneeva, Ulyanovsk State Univ. (Russian Federation)

Published in SPIE Proceedings Vol. 8221:
Optical Interactions with Tissue and Cells XXIII
E. Duco Jansen; Robert J. Thomas, Editor(s)

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