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Proceedings Paper

The observation of mitochondrial movement and ATG5 position in Arabidopsis during the process of infection with virulent and avirulent P. syringae strains
Author(s): Liu Yang; Chao Ma; Wen li Chen
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Paper Abstract

Infection of plants with pathogens leads to programmed cell death (PCD) associated with the pathogen-triggered hypersensitive response (HR) during plant innate immunity. In this study, the effects of infection by virulent Pseudomonas syringae pv. tomato (Pst) DC3000 and strains harboring avirulence factors AvrRps4 on the induction of HR-PCD were compared. We used Arabidopsis thaliana plants as materials, which expressed green fluorescent protein labeled mitochondria (mito-GFP) and green fluorescent protein tagged ATG5 (ATG5-GFP), these GFP are instantaneous expression. We found both Pst DC3000 and Pst-avrRps4 could induce mitochondria to assemble, the effect of Pst DC3000 was more obvious. ATG5 was located in chloroplasts after infection with Pst DC3000 or Pst-avrRps4. Under the condition of Pst-avrRps4, the expression of ATG5 was stronger than Pst DC3000 treatment.

Paper Details

Date Published: 2 February 2012
PDF: 8 pages
Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82331G (2 February 2012); doi: 10.1117/12.907215
Show Author Affiliations
Liu Yang, South China Normal Univ. (China)
Chao Ma, South China Normal Univ. (China)
Wen li Chen, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 8233:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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