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Proceedings Paper

Stokes vector formalism based second harmonic generation microscopy
Author(s): Jianjun Qiu; Nirmal Mazumder; Han-Ruei Tsai; Chih-Wei Hu; Fu-Jen Kao
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Paper Abstract

In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

Paper Details

Date Published: 13 February 2012
PDF: 8 pages
Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280C (13 February 2012); doi: 10.1117/12.907199
Show Author Affiliations
Jianjun Qiu, National Yang-Ming Univ. (Taiwan)
Nirmal Mazumder, National Yang-Ming Univ. (Taiwan)
Han-Ruei Tsai, National Yang-Ming Univ. (Taiwan)
Chih-Wei Hu, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)


Published in SPIE Proceedings Vol. 8228:
Single Molecule Spectroscopy and Superresolution Imaging V
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling; Ingo Gregor, Editor(s)

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