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Proceedings Paper

Exploring cytoplasmic dynamics in zebrafish yolk cells by single particle tracking of fluorescent nanodiamonds
Author(s): Cheng-Chun Chang; Bailin Zhang; Che-Yu Li; Chih-Chien Hsieh; Guillaume Duclos; François Treussart; Huan-Cheng Chang
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Paper Abstract

Fluorescent nanodiamonds (FNDs) have recently developed into an exciting new tool for bioimaging applications. The material possesses several unique features including high biocompatibility, easy bioconjugation, and perfect photostability, making it a promising optical nanoprobe in vitro as well as in vivo. This work explores the potential application of this novel nanomaterial as a photostable, nontoxic tracer in vivo using zebrafish as a model organism. We introduced FNDs into the yolk of a zebrafish embryo by microinjection at the 1-cell stage. Movements of the injected particles were investigated by using single particle tracking techniques. We observed unidirectional and stop-and-go traffic as part of the intricate cytoplasmic movements in the yolk cell. We determined a velocity in the range of 0.19 - 0.40 μm/s for 40 particles moving along with the axial streaming in the early developmental stage (1 to 2 hours post fertilization) of the zebrafish embryos.

Paper Details

Date Published: 24 February 2012
PDF: 8 pages
Proc. SPIE 8272, Advances in Photonics of Quantum Computing, Memory, and Communication V, 827205 (24 February 2012); doi: 10.1117/12.907181
Show Author Affiliations
Cheng-Chun Chang, Academia Sinica (Taiwan)
National Taiwan Univ. (Taiwan)
Bailin Zhang, Academia Sinica (Taiwan)
Che-Yu Li, Academia Sinica (Taiwan)
National Taiwan Normal Univ. (Taiwan)
Chih-Chien Hsieh, Academia Sinica (Taiwan)
National Taiwan Normal Univ. (Taiwan)
Guillaume Duclos, Ecole Normale Supérieure de Cachan (France)
François Treussart, Ecole Normale Supérieure de Cachan (France)
Lab. de Photonique Quantique et Moléculaire, CNRS (France)
Huan-Cheng Chang, Academia Sinica (Taiwan)
National Taiwan Univ. (Taiwan)
National Taiwan Normal Univ. (Taiwan)


Published in SPIE Proceedings Vol. 8272:
Advances in Photonics of Quantum Computing, Memory, and Communication V
Zameer Ul Hasan; Philip R. Hemmer; Hwang Lee; Charles M. Santori, Editor(s)

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