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Proceedings Paper

Milk phospholipid's protective effects against UV damage in skin equivalent models
Author(s): Carl Dargitz; Ashley Russell; Michael Bingham; Zyra Achay; Rafael Jimenez-Flores; Lily H. Laiho
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Paper Abstract

Exposure of skin tissue to UV radiation has been shown to cause DNA photodamage. If this damaged DNA is allowed to replicate, carcinogenesis may occur. DNA damage is prevented from being passed on to daughter cells by upregulation of the protein p21. p21 halts the cells cycle allowing the cell to undergo apoptosis, or repair its DNA before replication. Previous work suggested that milk phospholipids may possess protective properties against UV damage. In this study, we observed cell morphology, cell apoptosis, and p21 expression in tissue engineered epidermis through the use of Hematoxylin and Eosin staining, confocal microscopy, and western blot respectively. Tissues were divided into four treatment groups including: a control group with no UV and no milk phospholipid treatment, a group exposed to UV alone, a group incubated with milk phospholipids alone, and a group treated with milk phospholipids and UV. All groups were incubated for twenty-four hours after treatment. Tissues were then fixed, processed, and embedded in paraffin. Performing western blots resulted in visible p21 bands for the UV group only, implying that in every other group, p21 expression was lesser. Numbers of apoptotic cells were determined by observing the tissues treated with Hoechst dye under a confocal microscope, and counting the number of apoptotic and total cells to obtain a percentage of apoptotic cells. We found a decrease in apoptotic cells in tissues treated with milk phospholipids and UV compared to tissues exposed to UV alone. Collectively, these results suggest that milk phospholipids protect cell DNA from damage incurred from UV light.

Paper Details

Date Published: 13 February 2012
PDF: 9 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251V (13 February 2012); doi: 10.1117/12.907128
Show Author Affiliations
Carl Dargitz, California Polytechnic State Univ. (United States)
Ashley Russell, California Polytechnic State Univ. (United States)
Michael Bingham, California Polytechnic State Univ. (United States)
Zyra Achay, California Polytechnic State Univ. (United States)
Rafael Jimenez-Flores, California Polytechnic State Univ. (United States)
Lily H. Laiho, California Polytechnic State Univ. (United States)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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