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Proceedings Paper

Highly accurate measurement of varying drug dosage for real-time analysis of chemomechanical response of cardiomyocytes
Author(s): Avneet Bajwa; Behraad Bahreyni; Ash M. Parameswaran
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Paper Abstract

A recent advancement in the study of drug development for cardiovascular diseases is based on measuring the mechanical response of a single cardiomyocyte to various drug concentrations. This method requires delivering a specific dose of the drug over a short period of time while measuring the forces exerted by a cell that is kept inside a microchamber. However, the exact drug dosage is difficult to control for rapid variations in drug concentration, which hinders the accuracy of the measurements. This paper reports a highly sensitive technique for accurate and real-time measurement of minute variations in drug concentration. The fluid electrical conductivity is monitored using an array of electrodes along a micro-channel that eventually leads to the microchamber where the cardiomyocyte is placed. The microfluidic setup is fabricated through bonding of a moulded Polydimethylsiloxane (PDMS) layer to a glass substrate with patterned gold electrodes. The real-time differential measurements let us measure the local drug concentration with accuracies of better than 10pMol/mL. By using the data from all of the array electrodes, the profile of the drug plug as it travels along the microchannel from the injection point to the cell location can be derived with high precision. The multidomain numerical simulations of the microfluidic setup are in line with the measured experimental data. Our technique can be easily integrated into many existing and new designs thus providing a robust approach for label-free measurement of fluid properties in cell viability studies.

Paper Details

Date Published: 14 February 2012
PDF: 9 pages
Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510N (14 February 2012); doi: 10.1117/12.907105
Show Author Affiliations
Avneet Bajwa, Simon Fraser Univ. (Canada)
Behraad Bahreyni, Simon Fraser Univ. (Canada)
Ash M. Parameswaran, Simon Fraser Univ. (Canada)


Published in SPIE Proceedings Vol. 8251:
Microfluidics, BioMEMS, and Medical Microsystems X
Holger Becker; Bonnie L. Gray, Editor(s)

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