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Proceedings Paper

Time resolved optical biopsy spectroscopy of normal, benign and malignant tissues from NADH and FAD changes
Author(s): V. Masilamani; B. B. Das; J. Secor; M. AlSalhi; S. B. Amer; K. Farhat; D. Rabah; R. R. Alfano
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Paper Abstract

Histo pathological examination is the gold standard to discriminate between benign and malignant growth of tissue. But this is invasive and stressful. Hence many non invasive imaging techniques, such as CT, MRI, PET, etc are employed, each having certain advantages and disadvantages. In this context optical biopsy is a newly emerging technique, since it employs non-ionizing radiation like light or laser, which could be shined directly or launched through optical fiber to reach any part of the body. This paper reports results of time resolved emission spectra of 24 excised tissue sample (normal control=12; benign=4; malignant=8) of breast and prostate, employing a 390nm, 100 fs, Ti-Sapphire laser pulses. The fluorescence decay times were measured using streak camera and fitted for single and bi- exponential decays with reliability of 97%. Our results show the distinct difference between normal, benign and malignant tissues attributed changes of NADH and FAD levels.

Paper Details

Date Published: 31 January 2012
PDF: 7 pages
Proc. SPIE 8220, Optical Biopsy X, 822009 (31 January 2012); doi: 10.1117/12.906913
Show Author Affiliations
V. Masilamani, King Saud Univ. (Saudi Arabia)
B. B. Das, Fairfield Univ. (United States)
J. Secor, The City College of New York (United States)
M. AlSalhi, King Saud Univ. (Saudi Arabia)
S. B. Amer, King Faisal Specialist Hospital & Research Ctr. (Saudi Arabia)
K. Farhat, Princess Johara Al-Ibrahim Ctr. for Cancer Research (Saudi Arabia)
D. Rabah, Princess Johara Al-Ibrahim Ctr. for Cancer Research (Saudi Arabia)
R. R. Alfano, The City College of New York (United States)

Published in SPIE Proceedings Vol. 8220:
Optical Biopsy X
Robert R. Alfano; Stavros G. Demos, Editor(s)

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