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Proceedings Paper

Attenuation fluctuations and local dermal reflectivity are indicators of immune cell infiltrate and epidermal hyperplasia in skin inflammation
Author(s): Kevin G. Phillips; Yun Wang; Niloy Choudhury; David Levitz; Emily Swanzey; James Lagowski; Molly Kulesz-Martin; Steven Jacques
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Paper Abstract

Psoriasis is a common inflammatory skin disease resulting from genetic and environmental alterations of cutaneous immune responses responsible for skin homeostasis. While numerous therapeutic targets involved in the immunopathogenesis of psoriasis have been identified, the in vivo dynamics of psoriasis remains under investigated. To elucidate the spatial-temporal morphological evolution of psoriasis we undertook in vivo time course focus-tracked optical coherence tomography (OCT) imaging to non-invasively document dermal alterations due to immune cell infiltration and epidermal hyperplasia in an Imiquimod (IMQ) induced model of psoriasis-like inflammation in DBA2/C57Bl6 hybrid mice. Quantitative appraisal of dermal architectural changes was achieved through a three parameter fit of OCT axial scans in the dermis of the form A(z) = ρ exp(-mu;z +ε(z)). Ensemble averaging of the fit parameters over 2000 axial scans per mouse in each treatment arm revealed that the local dermal reflectivity ρ, decreased significantly in response to 6 day IMQ treatment (p = 0.0001), as did the standard deviation of the attenuation fluctuation std(ε(z)), (p = 0.04), in comparison to cream controls and day 1 treatments. No significant changes were observed in the average dermal attenuation rate, μ. Our results suggest these label-free OCT-based metrics can be deployed to investigate new therapeutic targets in animal models as well as aid in clinical staging of psoriasis in conjunction with the psoriasis area and severity index.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 820708 (9 February 2012); doi: 10.1117/12.906865
Show Author Affiliations
Kevin G. Phillips, Oregon Health & Science Univ. (United States)
Yun Wang, Oregon Health & Science Univ. (United States)
Niloy Choudhury, Oregon Health & Science Univ. (United States)
David Levitz, Oregon Health & Science Univ. (United States)
Emily Swanzey, Oregon Health & Science Univ. (United States)
James Lagowski, Oregon Health & Science Univ. (United States)
Molly Kulesz-Martin, Oregon Health & Science Univ. (United States)
Steven Jacques, Oregon Health & Science Univ. (United States)

Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory M.D.; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg M.D.; Melissa J. Suter; Brian Jet-Fei Wong M.D.; Justus F. Ilgner M.D.; Stephen Lam; Bodo E. Knudsen M.D.; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney M.D.; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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