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Proceedings Paper

Magnetic vesicles as MRI-trackable biogenic nanovectors
Author(s): Amanda K. Andriola Silva; Nathalie Luciani; Florence Gazeau; Claire Wilhelm
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Paper Abstract

Magnetic labeling renders cells MRI-detectable which provides attractive solutions for tracking the fate of a transplanted cell population. Understanding the interplay of magnetic nanoparticles and cells is then an important point that should not be neglected. Here we show that in the condition of food starvation, macrophage cells emit vesicles containing nanoparticles. First, we inferred the intracellular iron oxide load from the magnetophoretic velocity of cells at a calibrated magnetic field gradient. After magnetic labeling and culture in stress conditions, the intracellular iron oxide load was once more determined and a detectable difference was observed before and after stress. Moreover, we identified in the stress conditioned medium membrane vesicle structures carrying magnetic particles. Besides pointing out the role of cell-derived vesicles in the sequestration of the intracellular magnetic label, experiments also demonstrated that vesicles were able to chaperone the magnetic cargo into naïve cells.

Paper Details

Date Published: 2 February 2012
PDF: 7 pages
Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320Y (2 February 2012); doi: 10.1117/12.906783
Show Author Affiliations
Amanda K. Andriola Silva, Lab. Matière et Systèmes Complexes, CNRS, Univ. Paris 7-Denis Diderot (France)
Nathalie Luciani, Lab. Matière et Systèmes Complexes, CNRS, Univ. Paris 7-Denis Diderot (France)
Florence Gazeau, Lab. Matière et Systèmes Complexes, CNRS, Univ. Paris 7-Denis Diderot (France)
Claire Wilhelm, Lab. Matière et Systèmes Complexes, CNRS, Univ. Paris 7-Denis Diderot (France)


Published in SPIE Proceedings Vol. 8232:
Colloidal Nanocrystals for Biomedical Applications VII
Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski, Editor(s)

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