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Proceedings Paper

Three dimensional high-resolution imaging of lung preparations using ultramicroscopy
Author(s): D. Schwenninger; C. Dassow; J. Guttmann
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Paper Abstract

Ultramicroscopy allows imaging tissue three dimensionally with high optical resolution. To work, it requires optically clearing the tissue sample (e.g. by substitution of tissue-liquids with a liquid that reveals the same refractive index as the tissue's protein). In ultramicroscopy, illumination of the tissue with a thin sheet of light enables recording sectional images that can form a 3D-stack. Our preliminary data shows that it is feasible to create highly translucent lung tissue preparations of whole lungs with mechanical properties that are close to in vivo. We conclude that using this method, three dimensional image stacks of an entire lung can be recorded at different states of lung distension. This would ultimately help raising knowledge about fundamental lung function on the cellular and the alveolar level.

Paper Details

Date Published: 9 February 2012
PDF: 12 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82073I (9 February 2012); doi: 10.1117/12.906711
Show Author Affiliations
D. Schwenninger, Univ. Medical Ctr. Freiburg (Germany)
C. Dassow, Univ. Medical Ctr. Freiburg (Germany)
J. Guttmann, Univ. Medical Ctr. Freiburg (Germany)


Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg; Melissa J. Suter; Brian Jet-Fei Wong; Justus F. Ilgner; Stephen Lam; Bodo E. Knudsen; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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