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Proceedings Paper

Multiwavelength FLIM: new concept for fluorescence diagnosis
Author(s): Angelika Rück; S. Lorenz; Carmen Hauser; S. Mosch; S. Kalinina
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Paper Abstract

Fluorescence guided tumor resection is very well accepted in the case of bladder cancer and brain tumor, respectively. However, false positive results are one of the major problems, which will make the discrimination between tumor tissue and inflammation difficult. In contrast fluorescence lifetime imaging (FLIM) and especially spectral resolved FLIM (SLIM) can significantly improve the analysis. The fluorescence decay of a fluorophore in many cases does not show a simple monoexponential profile. A very complex situation arises, when more than one compound has to be analyzed. This could be the case when endogenous fluorophores of living cells and tissues have to be discriminated to identify oxidative metabolic changes. Other examples are PDT, when different photosensitizer metabolites are observed simultaneously. In those cases a considerable improvement could be achieved when time-resolved and spectral-resolved techniques are simultaneously incorporated. Within this presentation the principles of spectral and time-resolved fluorescence imaging will be discussed. Successful applications as autofluorescence and 5-ALA induced porphyrin fluorescence will be described in more detail.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260G (9 February 2012); doi: 10.1117/12.906620
Show Author Affiliations
Angelika Rück, Institute for Laser Technologies in Medicine and Metrology (Germany)
S. Lorenz, Institute for Laser Technologies in Medicine and Metrology (Germany)
Carmen Hauser, Institute for Laser Technologies in Medicine and Metrology (Germany)
S. Mosch, Institute for Laser Technologies in Medicine and Metrology (Germany)
S. Kalinina, Institute for Laser Technologies in Medicine and Metrology (Germany)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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