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Proceedings Paper

Effect of hormonal variation on in vivo high wavenumber Raman spectra improves cervical precancer detection
Author(s): Shiyamala Duraipandian; Wei Zheng; Joseph Ng; Jeffrey J. H. Low; A. Ilancheran; Zhiwei Huang
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Paper Abstract

Raman spectroscopy is a unique analytical probe for molecular vibration and is capable of providing specific spectroscopic fingerprints of molecular compositions and structures of biological tissues. The aim of this study is to improve the classification accuracy of cervical precancer by characterizing the variations in the normal high wavenumber (HW - 2800-3700cm-1) Raman spectra arising from the menopausal status of the cervix. A rapidacquisition near-infrared (NIR) Raman spectroscopic system was used for in vivo tissue Raman measurements at 785 nm excitation. Individual HW Raman spectrum was measured with a 5s exposure time from both normal and precancer tissue sites of 15 patients recruited. The acquired Raman spectra were stratified based on the menopausal status of the cervix before the data analysis. Significant differences were noticed in Raman intensities of prominent band at 2924 cm-1 (CH3 stretching of proteins) and the broad water Raman band (in the 3100-3700 cm-1 range) with a peak at 3390 cm-1 in normal and dysplasia cervical tissue sites. Multivariate diagnostic decision algorithm based on principal component analysis (PCA) and linear discriminant analysis (LDA) was utilized to successfully differentiate the normal and precancer cervical tissue sites. By considering the variations in the Raman spectra of normal cervix due to the hormonal or menopausal status of women, the diagnostic accuracy was improved from 71 to 91%. By incorporating these variations prior to tissue classification, we can significantly improve the accuracy of cervical precancer detection using HW Raman spectroscopy.

Paper Details

Date Published: 22 February 2012
PDF: 6 pages
Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 82140A (22 February 2012); doi: 10.1117/12.906399
Show Author Affiliations
Shiyamala Duraipandian, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Joseph Ng, National Univ. Hospital (Singapore)
National Univ. of Singapore (Singapore)
Jeffrey J. H. Low, National Univ. Hospital (Singapore)
National Univ. of Singapore (Singapore)
A. Ilancheran, National Univ. Hospital (Singapore)
National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 8214:
Advanced Biomedical and Clinical Diagnostic Systems X
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

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