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Proceedings Paper

Facile synthesis of fluorescent gold nanoclusters and their application in cellular imaging
Author(s): Li Shang; René M. Dörlich; Stefan Brandholt; Naghmeh Azadfar; Gerd Ulrich Nienhaus
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Paper Abstract

Fluorescent metal nanoclusters (NCs) have many attractive features, including ultrasmall size, good biocompatibility and large Stokes shift, thus making them promising alternatives as fluorescent markers for biological applications. We have developed a facile method of synthesizing water-soluble, near-infrared (NIR) luminescent AuNCs. By virtue of the long fluorescence lifetime of these AuNCs, we explored their application in cellular fluorescence lifetime imaging (FLIM). Furthermore, the interaction of AuNCs with biomolecules such as proteins has been investigated. Upon protein association, the fluorescence of AuNCs was found to be enhanced and their luminescence lifetime was prolonged. Our studies underline the importance of studying the behavior of AuNCs within the complex biological environment for their application as novel cellular imaging probes.

Paper Details

Date Published: 2 February 2012
PDF: 7 pages
Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82321J (2 February 2012); doi: 10.1117/12.906250
Show Author Affiliations
Li Shang, Karlsruher Institut für Technologie (Germany)
René M. Dörlich, Karlsruher Institut für Technologie (Germany)
Stefan Brandholt, Karlsruher Institut für Technologie (Germany)
Naghmeh Azadfar, Karlsruher Institut für Technologie (Germany)
Gerd Ulrich Nienhaus, Karlsruher Institut für Technologie (Germany)
Univ. of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 8232:
Colloidal Nanocrystals for Biomedical Applications VII
Wolfgang J. Parak; Kenji Yamamoto M.D.; Marek Osinski, Editor(s)

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