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Proceedings Paper

In vivo imaging of cell nuclei by photoacoustic microscopy without staining
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Paper Abstract

Ultraviolet photoacoustic microscopy (UVPAM) can image cell nuclei in vivo with high contrast and resolution noninvasively without staining. Here, we used UV light at wavelengths of 210-310 nm for excitation of DNA and RNA to produce photoacoustic waves. We applied the UVPAM to in vivo imaging of cell nuclei in mouse skin, and obtained UVPAM images of the unstained cell nuclei at wavelengths of 245-282 nm as ultrasound gel was used for acoustic coupling. The largest ratio of contrast to noise was found for the images of cell nuclei at a 250 nm wavelength.

Paper Details

Date Published: 23 February 2012
PDF: 6 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82231X (23 February 2012); doi: 10.1117/12.906211
Show Author Affiliations
Da-Kang Yao, Washington Univ. in St. Louis (United States)
Ruimin Chen, The Univ. of Southern California (United States)
Konstantin Maslov, Washington Univ. in St. Louis (United States)
Qifa Zhou, The Univ. of Southern California (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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