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Proceedings Paper

Photoacoustic microscopy of myocardial sheet architecture in unfixed and unstained mammalian hearts
Author(s): Chi Zhang; Ya-Jian Cheng; Da-Kang Yao; Samuel Wickline; Lihong V. Wang
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Paper Abstract

The laminar myocardial sheet architecture and its dynamic change play a key role in myocardial wall thickening. Histology, confocal optical microscopy (COM), and diffusion tensor MRI (DTI) have been used to unveil the structures and functions of the myocardial sheets. However, histology and COM require fixation, sectioning, and staining processes, which dehydrate and deform the sheet architecture. Although DTI can delineate sheet architecture nondestructively in viable hearts, it cannot provide cellular-level resolution. Here we show that photoacoustic microscopy (PAM), with high resolution (~1 μm) and label-free detection, is appropriate for imaging 3D myocardial architecture. Perfused half-split mouse hearts were also imaged by PAM in vitro without fixation, dehydration, nor staining. The laminar myocardial sheet architecture was clearly visualized within a 0.15 mm depth range. Two populations of oppositely signed sheet angles were observed. Therefore, PAM promises to access dynamic changes of myocardial architectures in ex vivo perfused-viable hearts.

Paper Details

Date Published: 23 February 2012
PDF: 5 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82232G (23 February 2012); doi: 10.1117/12.906208
Show Author Affiliations
Chi Zhang, Washington Univ. in St. Louis (United States)
Ya-Jian Cheng, Washington Univ. School of Medicine in St. Louis (United States)
Da-Kang Yao, Washington Univ. in St. Louis (United States)
Samuel Wickline, Washington Univ. School of Medicine in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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