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Proceedings Paper

Label-free photoacoustic microscopy of cytochrome c in cells
Author(s): Chi Zhang; Yu Zhang; Da-Kang Yao; Younan Xia; Lihong V. Wang
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Paper Abstract

Cytochrome c is a heme protein normally bound to mitochondria and is important for mitochondrial electron transport and apoptosis initiation. Since cytochrome c is nonfluorescent, it is always labeled with fluorescent molecules for imaging, which, however, may affect normal cellular functions. Here, label-free photoacoustic microscopy (PAM) of mitochondrial cytochrome c was realized for the first time by utilizing the optical absorption around the Soret peak. PAM was demonstrated to be sensitive enough to image mitochondrial cytochrome c at 422 nm wavelength. Mitochondrial cytochrome c in the cytoplasm of fixed fibroblasts was clearly imaged by PAM as confirmed by fluorescent labeling. By showing mitochondrial cytochrome c in various cells, we demonstrated the feasibility of PAM for label-free histology of mouse ear sections. Therefore, PAM can sensitively image cytochrome c in unstained cells at 422 nm wavelength and has great potential for functional imaging of cytochrome c in live cells or in vivo.

Paper Details

Date Published: 23 February 2012
PDF: 5 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82231W (23 February 2012); doi: 10.1117/12.906202
Show Author Affiliations
Chi Zhang, Washington Univ. in St. Louis (United States)
Yu Zhang, Washington Univ. in St. Louis (United States)
Da-Kang Yao, Washington Univ. in St. Louis (United States)
Younan Xia, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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