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Proceedings Paper

Heavy metal stress detection and monitoring via LED-induced chlorophyll fluorescence analysis of Zea mays L. seedlings aimed at polluted soil phytoremediation
Author(s): Artur S. Gouveia-Neto; Elias A. Silva; Airon José da Silva; Clístenes W. A. do Nascimento
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Paper Abstract

Chlorophyll fluorescence spectroscopy is employed to detect and study the time evolution of metal stress of Zea mays L. seedlings aiming polluted soil phytoremediation. The chlorophyll fluorescence spectra of intact leaves are analyzed using 405 nm LED excitation. Red (Fr) and far-red (FFr) emissions around 685 nm and 735 nm, respectively, are examined as a function of the heavy metal concentration. The fluorescence ratio Fr/FFr was employed to monitor the effect of heavy metal upon the physiological state of the plants before signs of visual stress became apparent. The chlorophyll fluorescence analysis permitted detection and evaluation of the damage caused by heavy metal soil contamination in the early stages of the plants growing process, which is not feasible using conventional in vitro spectral analysis.

Paper Details

Date Published: 9 February 2012
PDF: 9 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 822505 (9 February 2012); doi: 10.1117/12.905456
Show Author Affiliations
Artur S. Gouveia-Neto, Univ. Federal Rural de Pernambuco (Brazil)
Elias A. Silva, Univ. Federal Rural de Pernambuco (Brazil)
Airon José da Silva, Univ. Federal Rural de Pernambuco (Brazil)
Clístenes W. A. do Nascimento, Univ. Federal Rural de Pernambuco (Brazil)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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